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金黄色葡萄球菌和大肠杆菌中四环素抗性蛋白的分离与鉴定

Isolation and characterization of tetracycline resistance proteins from Staphylococcus aureus and Escherichia coli.

作者信息

Wojdani A, Avtalion R R, Sompolinsky D

出版信息

Antimicrob Agents Chemother. 1976 Mar;9(3):526-34. doi: 10.1128/AAC.9.3.526.

DOI:10.1128/AAC.9.3.526
PMID:769681
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC429564/
Abstract

Immunoglobulin (adsorbed resistance antiserum) reacting specifically with antigens from tetracycline (Tc)-resistant Staphylcoccus aureus or Escherichia coli was produced by adsorbing immunoglobulin against cell envelopes of resistant strains with envelope extracts from the respective isogenic susceptible strains. Adsorbed resistance antiserum against S. aureus reacted with envelope extracts from 32 Tc-resistant strains and failed to react with similar extracts from 76 Tc-susceptible strains of S. aureus. An antigen (Tc resistance antigen [TRA]) found only in Tc-resistant strains was produced by adsorbing envelope extracts from these strains with immunoglobulin against envelopes from isogenic Tc-susceptible strains. On immunodiffusion no cross-reactivity between TRAs from S. aureus and E. coli was observed. The TRAs behaved as proteins. The molecular weight of TRA from S. aureus was determined to be 32,000 and from E. coli to be 50,000. Data obtained by preliminary amino acid analysis of the TRAs are presented.

摘要

通过用来自相应同基因敏感菌株的包膜提取物吸附针对抗性菌株细胞包膜的免疫球蛋白，制备了与四环素（Tc）抗性金黄色葡萄球菌或大肠杆菌的抗原特异性反应的免疫球蛋白（吸附抗性抗血清）。针对金黄色葡萄球菌的吸附抗性抗血清与32株Tc抗性菌株的包膜提取物反应，而不与76株Tc敏感金黄色葡萄球菌的类似提取物反应。通过用针对同基因Tc敏感菌株包膜的免疫球蛋白吸附这些菌株的包膜提取物，产生了仅在Tc抗性菌株中发现的一种抗原（Tc抗性抗原[TRA]）。在免疫扩散中，未观察到金黄色葡萄球菌和大肠杆菌的TRA之间的交叉反应。TRA表现为蛋白质。确定来自金黄色葡萄球菌的TRA的分子量为32,000，来自大肠杆菌的TRA的分子量为50,000。本文给出了TRA初步氨基酸分析获得的数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/71f6cc786065/aac00309-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/dfe008a4a7da/aac00309-0177-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/71f6cc786065/aac00309-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/dfe008a4a7da/aac00309-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/58a2280c1cb7/aac00309-0177-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/8fc41217542e/aac00309-0177-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/c8000a9dd916/aac00309-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/baf1702449d2/aac00309-0178-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/67b59b2a503d/aac00309-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/baa380035225/aac00309-0180-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a1b/429564/71f6cc786065/aac00309-0181-a.jpg

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引用本文的文献

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本文引用的文献

1
Antigen specific for bacteria resistant to tetracycline.
Antimicrob Agents Chemother. 1974 Aug;6(2):117-20. doi: 10.1128/AAC.6.2.117.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
细菌对四环素的耐药性。
Microbiol Rev. 1978 Dec;42(4):707-24. doi: 10.1128/mr.42.4.707-724.1978.
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Inducible resistance to D-cycloserine in Bacillus subtilis 168.枯草芽孢杆菌168中对D-环丝氨酸的诱导抗性
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Mechanism of tetracycline resistance in Staphylococcus aureus.金黄色葡萄球菌中四环素耐药性的机制。
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Transferable resistance factors with mutator effect in Salmonella typhi.
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Depressed resistance to tetracycline in Staphylococcus aureus.金黄色葡萄球菌对四环素的耐药性降低。
Microbios. 1971 Mar;3(10):165-80.
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J Gen Microbiol. 1973 Dec;79(2):343-5. doi: 10.1099/00221287-79-2-343.
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