Popa L M, Winter S, Löber G
Medical and Pharmaceutical University of Cluj-Napoca, Romania.
Biochem Mol Biol Int. 1994 Dec;34(6):1189-96.
The DNA bis-intercalator oxazole homodimer (YOYO-3: 1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-methy l- 2,3-dihydro-(benzo-1,3-oxazole)-2-methylidene]-quinolinium tetraiodide) specifically alters the electrophoretic pattern of covalently closed circular (CCC) DNA molecules. Thus, YOYO-3 seems to remove the CCC DNA supercoils and induces the appearance of additional bands by changing the linking number. It also promotes an unusual "star" activity for the restriction enzyme Hind III. Fluorescence lifetime measurements indicate that YOYO-3 is capable of binding to DNA by bis- and mono-intercalation.
DNA双嵌入剂恶唑同二聚体（YOYO-3：1,1'-（4,4,7,7-四甲基-4,7-二氮杂十一亚甲基）-双-4-[3-甲基-2,3-二氢-（苯并-1,3-恶唑）-2-亚甲基]-喹啉鎓四碘化物）能特异性改变共价闭合环状（CCC）DNA分子的电泳图谱。因此，YOYO-3似乎能消除CCC DNA超螺旋，并通过改变连接数诱导出现额外的条带。它还能促进限制性内切酶Hind III产生异常的“星号”活性。荧光寿命测量表明，YOYO-3能够通过双嵌入和单嵌入与DNA结合。
