Corrigall A V, Hift R J, Adams P A, Kirsch R E
UCT/MRC Liver Research Centre, Department of Medicine, South Africa.
Biochem Mol Biol Int. 1994 Dec;34(6):1283-9.
We have studied the kinetics of the inhibition of mitochondrial protoporphyrinogen oxidase (PPO) from liver and placenta of 3 mammalian species by the diphenyl ether herbicide acifluorfen (AF). AF competitively inhibited PPO from human liver and placenta, mouse liver and pig placenta with respect to its substrate protoporphyrinogen. In contrast, mixed-type inhibition was shown for pig liver. The differing results shown in pig liver may point to structural differences in PPO derived from different species and tissues. We have also compared the effects of AF on the function of PPO in human lymphoblasts from normal subjects and those with variegate porphyria, an inherited disorder of PPO. Competitive inhibition was shown for both and there were no significant differences in the values of Ks or Ki.
我们研究了二苯醚除草剂三氟羧草醚（AF）对3种哺乳动物肝脏和胎盘线粒体原卟啉原氧化酶（PPO）的抑制动力学。相对于底物原卟啉原，AF对人肝脏和胎盘、小鼠肝脏及猪胎盘的PPO具有竞争性抑制作用。相比之下，猪肝脏表现出混合型抑制作用。猪肝脏中呈现的不同结果可能表明不同物种和组织来源的PPO存在结构差异。我们还比较了AF对正常受试者和患有迟发性皮肤卟啉症（一种PPO遗传性疾病）的人淋巴母细胞中PPO功能的影响。两者均表现出竞争性抑制作用，且Ks或Ki值无显著差异。
