Schenk T, Schuphan I, Schmidt B
Rheinisch-Westfälische Technische Hochschule Aachen, Institut für Biologie V, Germany.
J Chromatogr A. 1995 Feb 17;693(1):7-13. doi: 10.1016/0021-9673(94)01127-z.
The bacterial biosurfactants 3-[3'-(L-rhamnopyranosyloxy)decanoyloxy]decanoic acid (RL-1) and 3-[3'-(2"-O-alpha-L-rhamnopyranosyl-alpha-L-rhamnopyranosyloxy) decanoyloxy]decanoic acid (RL-2) were isolated from Pseudomonas aeruginosa DSM 2659 cultures. An HPLC method was developed for the p-bromophenacyl esters of the rhamnolipids. Separation was obtained within 25 min on a RP C18 column using a linear gradient of water-acetonitrile (30:70 to 0:100) and UV detection (265 and 320 nm). Linearity of response existed for 1.2-25 microg of the RL-1 and 1.8-25 microg of the RL-2-p-bromophenacyl ester (0.9-19.2 microg RL-1 and 1.3-18.0 microg RL-2). The reproducibility of the entire analytical method (extraction, derivatisation) was tested.
从铜绿假单胞菌DSM 2659培养物中分离出细菌生物表面活性剂3-[3'-(L-鼠李糖吡喃糖氧基)癸酰氧基]癸酸(RL-1)和3-[3'-(2''-O-α-L-鼠李糖吡喃糖基-α-L-鼠李糖吡喃糖氧基)癸酰氧基]癸酸(RL-2)。建立了一种用于鼠李糖脂对溴苯甲酰酯的高效液相色谱法。使用水-乙腈线性梯度(30:70至0:100)和紫外检测(265和320 nm)，在RP C18柱上25分钟内实现分离。RL-1的1.2 - 25微克和RL-2对溴苯甲酰酯的1.8 - 25微克(0.9 - 19.2微克RL-1和1.3 - 18.0微克RL-2)存在线性响应。测试了整个分析方法(提取、衍生化)的重现性。
