水稻质膜H(+) -ATPase基因家族的鉴定与特征分析
Identification and characterization of a family of genes for the plasma membrane H(+)-ATPase of Oryza sativa L.
作者信息
Ookura T, Wada M, Sakakibara Y, Jeong K H, Maruta I, Kawamura Y, Kasamo K
机构信息
National Food Research Institute, Ibaraki, Japan.
出版信息
Plant Cell Physiol. 1994 Dec;35(8):1251-6. doi: 10.1093/oxfordjournals.pcp.a078720.
Abstract
A cDNA clone (cOSA2) encoding a plasma membrane H(+)-ATPase was isolated from rice. Southern blot analysis indicated that the genes that corresponds to cOSA2 was different from that to cOSA1. Northern blots revealed OSA2 mRNA in roots, calli and shoots. OSA1 transcripts were detected only by RT-PCR in these tissues.
摘要
从水稻中分离出一个编码质膜H(+) -ATP酶的cDNA克隆（cOSA2）。Southern杂交分析表明，与cOSA2对应的基因与cOSA1对应的基因不同。Northern杂交显示在根、愈伤组织和芽中有OSA2 mRNA。在这些组织中，仅通过RT-PCR检测到OSA1转录本。
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