Sulkowski S, Chyczewski L, Dziecioł J, Sulkowska M, Kozielec Z
Department of Pathological Anatomy, Medical Academy, Białystok.
Pol J Pathol. 1994;45(4):299-302.
Quantitative and morphological analyses (in SEM) of blood platelets collected from the left and right ventricles of the rat heart in the course of experimental lung emphysema were done. Platelet aggregation index was estimated, too. Emphysema was induced by a single intratracheal instillation of papain solution in a dose of 20 mg/kg b.w/1 ml PBS. The animals were sacrificed after 2 and 24 hours and 7, 14, 28 days later. Within 24 hours of the experiment a slight decrease was observed in the number of platelets in the blood collected from the left ventricle compared to the right one as well as to control animals. Also a reduction in platelet aggregation coefficient value was noted. However, in the later period of emphysema progression (after 7th day), a statistically significant increase was found in the number of blood platelets in the left ventricle. A relation was noted between quantitative changes of blood platelets and emphysema progression evaluated morphometrically. The ultrastructural examinations in SEM suggest the occurrence of platelet satellitosis in animals intratracheally injected with papain solution. The present results indicate the possibility of a significant contribution of blood platelets to the pathogenesis of experimental lung emphysema.
对实验性肺气肿大鼠心脏左、右心室采集的血小板进行了定量和形态学分析（扫描电子显微镜下）。还估算了血小板聚集指数。通过气管内单次滴注剂量为20mg/kg体重/1ml PBS的木瓜蛋白酶溶液诱导肺气肿。在2小时、24小时以及7天、14天、28天后处死动物。实验24小时内，与右心室以及对照动物相比，从左心室采集的血液中血小板数量略有减少。还注意到血小板聚集系数值降低。然而，在肺气肿进展后期（第7天后），左心室中血小板数量出现统计学上的显著增加。观察到血小板的定量变化与通过形态计量学评估的肺气肿进展之间存在关联。扫描电子显微镜下的超微结构检查表明，气管内注射木瓜蛋白酶溶液的动物出现了血小板卫星现象。目前的结果表明血小板对实验性肺气肿发病机制有显著作用的可能性。
