Zaczek M, Kedra D, Nowak K, Chłap Z
Department of Pathophysiology, Jagellonian University Medical College, Kraków.
Pol J Pathol. 1994;45(4):303-7.
The aim of this study was to establish the diagnostical value of nuclear area in proliferative lesions of colonic and bronchial epithelium. The study included 11 adenomas and 9 adenocarcinomas of colon, 11 control samples of normal colonic epithelium as well as 5 cases of bronchial squamous cell metaplasia, 25 squamous cell carcinomas, 9 small cell carcinomas and 3 adenocarcinomas of bronchi and 13 control samples of normal bronchial epithelium. All tissue samples were formalin fixed, paraffin embedded and silver stained. Morphometrical analysis was performed using optical Axiophot microscope with immersion, B/W CCD camera connected to frame grabber card, computer PC AT 386 and morphometrical software Vist and Morpho. Nuclear area was measured semiautomatically. Within each case 100 nuclei were analyzed. Statistically significant differences between carcinoma and adenoma or normal epithelium were observed in colon. In bronchi nuclear area was significantly higher in squamous cell carcinoma and adenocarcinoma if compared with other lesions including small cell carcinoma or bronchial metaplasia and normal epithelium.
本研究的目的是确定核面积在结肠和支气管上皮增生性病变中的诊断价值。该研究包括11例结肠腺瘤和9例结肠腺癌、11例正常结肠上皮对照样本，以及5例支气管鳞状上皮化生、25例鳞状细胞癌、9例小细胞癌和3例支气管腺癌，还有13例正常支气管上皮对照样本。所有组织样本均经福尔马林固定、石蜡包埋和银染色。使用配备油浸物镜的光学Axiophot显微镜、连接至图像采集卡的黑白CCD相机、PC AT 386计算机和形态测量软件Vist及Morpho进行形态测量分析。核面积采用半自动测量。在每个病例中分析100个细胞核。在结肠中，癌组织与腺瘤或正常上皮之间观察到统计学上的显著差异。在支气管中，与包括小细胞癌或支气管化生及正常上皮在内的其他病变相比，鳞状细胞癌和腺癌的核面积显著更高。
