Thibault N
Institut de Recherches Internationales Servier, Courbevoie, France.
Cell Biol Toxicol. 1994 Dec;10(5-6):323-8. doi: 10.1007/BF00755778.
Scanning laser cytometry, an analytic technique that provides an accurate fluorescent measurement in adherent cells, was used to study cholestatic mechanisms in isolated rat hepatocyte couplets (IRHC). Treatment of IRHC with cholestatic compounds induced a pericanalicular F-actin accumulation and an increase in cytosolic free calcium. These data obtained with a scanning cytometer used in conjunction with an in vitro model representing the primary secretory unit suggest that abnormalities of pericanalicular F-actin filaments and calcium homeostasis play a key role in cholestasis. Considering the necessity for the development of mechanistic studies in toxicology, this technique should prove to be an outstanding tool.
扫描激光细胞术是一种能对贴壁细胞进行精确荧光测量的分析技术，用于研究分离的大鼠肝小叶（IRHC）中的胆汁淤积机制。用胆汁淤积化合物处理IRHC会导致胆小管周围F-肌动蛋白积聚以及细胞溶质游离钙增加。这些数据是通过扫描细胞仪结合代表主要分泌单位的体外模型获得的，表明胆小管周围F-肌动蛋白丝和钙稳态异常在胆汁淤积中起关键作用。考虑到毒理学中开展机制研究的必要性，这项技术应被证明是一种出色的工具。
