Higashi N, Yoshizuka N, Ohuchi A, Osawa T, Kobayashi Y
Kao Institute for Fundamental Research, Tochigi, Japan.
Cell Immunol. 1995 Apr 1;161(2):288-94. doi: 10.1006/cimm.1995.1038.
We previously reported the amino acid sequence of the human macrophage chemotactic factor with a molecular weight of 1000 (MCF-1) was WLGREDGSE. In this study we examined the roles of MCF-1 and tumor necrosis factor (TNF) in a guinea pig delayed-type hypersensitivity (DTH) reaction. When macrophage chemotactic activity (MCA) in a skin extract was subjected to gel filtration, only the activity with a molecular weight of 1000 was significantly inhibited by anti-human MCF-1 mAb (7-3 mAb). Both MCA and TNF activity were detected in skin extracts from DTH inflammation sites where the specific antigen was injected. Both activities were produced at the early DTH reaction stage. In contrast, MCF-1 was produced at the late DTH reaction stage. The erythema was suppressed significantly either by anti-guinea pig TNF Ab or by 7-3 mAb, suggesting the roles of these two cytokines in a DTH reaction.
我们之前报道过，分子量为1000的人巨噬细胞趋化因子（MCF-1）的氨基酸序列为WLGREDGSE。在本研究中，我们检测了MCF-1和肿瘤坏死因子（TNF）在豚鼠迟发型超敏反应（DTH）中的作用。当对皮肤提取物中的巨噬细胞趋化活性（MCA）进行凝胶过滤时，只有分子量为1000的活性被抗人MCF-1单克隆抗体（7-3单克隆抗体）显著抑制。在注射了特异性抗原的DTH炎症部位的皮肤提取物中检测到了MCA和TNF活性。这两种活性均在DTH反应早期产生。相比之下，MCF-1在DTH反应后期产生。抗豚鼠TNF抗体或7-3单克隆抗体均可显著抑制红斑，表明这两种细胞因子在DTH反应中发挥作用。
