Diener B, Becker R, Martus H J, Traiser M, Steinberg P, Oesch F
Institute of Toxicology, University of Mainz, Germany.
Carcinogenesis. 1995 Mar;16(3):633-6. doi: 10.1093/carcin/16.3.633.
Isolated rat liver parenchymal cells (PC) were co-cultured with a non-parenchymal rat liver epithelial cell line (NEC) or with an oval cell line. The homotypical gap junctional intercellular communication (GJIC) between the liver PC was measured after microinjection of Lucifer Yellow by dye transfer. The rat liver PC were dye coupled between 87% and 100% for at least 1 week in both co-cultures, in contrast to PC In monoculture between which no dye coupling was left after 1 week. When liver PC were co-cultured with a transformed and tumorigenic NEC or with a transformed and tumorigenic oval cell line the homotypical GJIC between the liver PC was drastically decreased with culture time, and the PC were then compressed and displaced by the expansive growth of the transformed cell lines. The disturbance of the GJIC between normal cells by adjacent tumorigenic cells might be a new mechanism to explain the expansive growth of tumors.
将分离的大鼠肝实质细胞（PC）与大鼠非实质肝上皮细胞系（NEC）或卵圆细胞系共培养。通过荧光黄注射后的染料转移来测量肝PC之间的同型间隙连接细胞间通讯（GJIC）。在两种共培养中，大鼠肝PC之间的染料偶联率在87%至100%之间，至少持续1周，相比之下，在单一培养中，1周后PC之间就不再有染料偶联。当肝PC与转化的致瘤性NEC或转化的致瘤性卵圆细胞系共培养时，随着培养时间的延长，肝PC之间的同型GJIC急剧下降，随后PC被转化细胞系的扩张性生长压缩和取代。相邻致瘤细胞对正常细胞间GJIC的干扰可能是解释肿瘤扩张性生长的一种新机制。
