Nishina Y, Sumi T, Iwai S A, Nishimune Y
Research Institute for Microbial Diseases, Dental Faculty, Osaka University, Japan.
Exp Cell Res. 1995 Apr;217(2):288-93. doi: 10.1006/excr.1995.1089.
Okadaic acid, a protein phosphatase inhibitor, is a strong tumor promoter which apparently activates protein phosphorylation. To examine the role of protein phosphatases in stem cell growth and differentiation, embryonal carcinoma F9 cells were treated with okadaic acid. In the presence of this agent, the cells showed rapid morphological changes and arrest of proliferation at the M phase of the cell cycle, accompanied by a marked increase in the mRNA expression of various differentiation markers. Okadaic acid induced rapid increase in the mRNA levels of both c-jun and junB and results indicate that the inhibition of phosphatase by okadaic acid induces apparent activation of protein phosphorylation and may cause the expression of differentiation marker genes in F9 cells via the activation of AP-1.
冈田酸是一种蛋白磷酸酶抑制剂，是一种强力肿瘤促进剂，它显然能激活蛋白磷酸化。为了研究蛋白磷酸酶在干细胞生长和分化中的作用，用冈田酸处理胚胎癌F9细胞。在这种试剂存在的情况下，细胞显示出快速的形态变化，并在细胞周期的M期停止增殖，同时各种分化标志物的mRNA表达显著增加。冈田酸诱导c-jun和junB的mRNA水平迅速升高，结果表明冈田酸对磷酸酶的抑制诱导了蛋白磷酸化的明显激活，并可能通过激活AP-1导致F9细胞中分化标志物基因的表达。
