Holt S J, Alexander P, Inman C B, Davies D E
CRC Medical Oncology Unit, Southampton General Hospital, United Kingdom.
Exp Cell Res. 1995 Apr;217(2):554-8. doi: 10.1006/excr.1995.1122.
Ligand-induced translocation of epidermal growth factor receptors (EGF-R) to the nucleus of NR6/HER fibroblasts has been studied by immunoelectron microscopy. Following treatment of NR6/HER cells with epidermal growth factor (EGF) for 1 h, there was a decrease in EGF-R labeling at the plasma membrane and a corresponding increase in EGF-R in the nucleus. This was preceded by a rapid and sustained increase in nuclear phosphotyrosine content, detectable within 2 min of EGF treatment. EGF-R translocation into the nucleus was completely prevented by 18 h serum starvation prior to treatment with EGF. These results indicate that translocation of EGF-R to the nucleus is a controlled process and they suggest that EGF-R may directly influence nuclear function.
通过免疫电子显微镜研究了配体诱导的表皮生长因子受体（EGF-R）向NR6/HER成纤维细胞核内的转位。用表皮生长因子（EGF）处理NR6/HER细胞1小时后，质膜上的EGF-R标记减少，而细胞核内的EGF-R相应增加。在此之前，核磷酸酪氨酸含量迅速且持续增加，在EGF处理后2分钟内即可检测到。在用EGF处理之前，18小时的血清饥饿可完全阻止EGF-R向细胞核的转位。这些结果表明，EGF-R向细胞核的转位是一个可控过程，提示EGF-R可能直接影响核功能。
