Thompson D B, Janssen R C, Ossowski V M, Prochazka M, Knowler W C, Bogardus C
Phoenix Epidemiology and Clinical Research Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Phoenix, Arizona 85016.
Diabetes. 1995 Apr;44(4):478-81. doi: 10.2337/diab.44.4.478.
A low acute insulin response (AIR) is a predictor of non-insulin-dependent diabetes mellitus (NIDDM) in insulin-resistant Pima Indians. We have initiated a search for regions of the genome linked with the AIR using sib-pair linkage analysis as a first step in identifying genes that are determinants of this phenotype. Eighteen short tandem-repeat polymorphisms from chromosome 1 were genotyped in over 900 Pima Indians and tested for linkage with NIDDM and in a subset of Pima Indians for linkage with AIR. The anonymous DNA marker D1S198 on chromosome 1p was linked with AIR (P = 0.000056) in 175 sib pairs from 60 families, all with normal glucose tolerance, but no linkage was observed between D1S198 and NIDDM (P = 0.44, 996 sib pairs). Additional markers genotyped on chromosome 1 did not show linkage with AIR or NIDDM. This study indicates that a locus on chromosome 1p may be a determinant of the phenotypic variation seen in the AIR.
低急性胰岛素反应（AIR）是胰岛素抵抗的皮马印第安人中非胰岛素依赖型糖尿病（NIDDM）的一个预测指标。我们已开始利用同胞对连锁分析寻找与AIR相关的基因组区域，作为鉴定决定该表型的基因的第一步。对来自900多名皮马印第安人的18个1号染色体短串联重复多态性进行基因分型，并测试其与NIDDM的连锁关系，在一部分皮马印第安人中测试其与AIR的连锁关系。1号染色体上的匿名DNA标记D1S198在来自60个家庭的175对同胞对中与AIR连锁（P = 0.000056），所有这些同胞对的糖耐量均正常，但未观察到D1S198与NIDDM之间的连锁关系（P = 0.44，996对同胞对）。在1号染色体上进行基因分型的其他标记未显示与AIR或NIDDM连锁。这项研究表明，1号染色体短臂上的一个基因座可能是AIR中所见表型变异的一个决定因素。
