Raulf F, Pérez J, Hoyer D, Bruns C
Preclinical Research, Sandoz Pharma Ltd., Basle, Switzerland.
Digestion. 1994;55 Suppl 3:46-53. doi: 10.1159/000201201.
Somatostatin regulates endocrine and exocrine secretion, acts as a neurotransmitter/neuro-modulator and possesses antiproliferative properties. These diverse physiological effects are mediated by G-protein coupled receptors of which at least five subtypes have been cloned (SSTR1-5). Here, we have investigated the tissue distribution pattern of mRNAs encoding the five SRIF receptor subtypes in the adult rat by RT-PCR analysis and in situ hybridization histochemistry. All five receptor subtypes were found to be expressed simultaneously in brain and pituitary by RT-PCR. Besides, the in situ hybridization results clearly show a distinct but overlapping expression pattern of SSTR1-5 mRNA in the central nervous system as was found by RT-PCR for the periphery. Such distinct SRIF receptor expression may contribute to the selective biological functions of the receptor subtypes.
生长抑素调节内分泌和外分泌分泌，作为一种神经递质/神经调节剂，并具有抗增殖特性。这些多样的生理效应由G蛋白偶联受体介导，其中至少五种亚型已被克隆（SSTR1 - 5）。在此，我们通过逆转录聚合酶链反应（RT-PCR）分析和原位杂交组织化学研究了成年大鼠中编码五种生长抑素释放抑制因子（SRIF）受体亚型的mRNA的组织分布模式。通过RT-PCR发现，所有五种受体亚型在脑和垂体中同时表达。此外，原位杂交结果清楚地显示，SSTR1 - 5 mRNA在中枢神经系统中的表达模式独特但有重叠，这与RT-PCR在外周组织中发现的情况相同。这种独特的SRIF受体表达可能有助于受体亚型的选择性生物学功能。
