来自不同纤维溶解丁酸弧菌菌株的限制性内切酶Bfi57I和Bfi89I的部分纯化及特性分析
Partial purification and characterisation of Bfi57I and Bfi89I, restriction endonucleases from different strains of Butyrivibrio fibrisolvens.
作者信息
Mohn W W, Teather R M
机构信息
Centre for Food and Animal Research, Agriculture Canada, Ottawa, Ontario.
出版信息
Gene. 1995 Mar 21;155(1):131-2. doi: 10.1016/0378-1119(94)00689-p.
Two class-II restriction endonucleases (ENases), Bfi57I and Bfi89I, were partially purified from Butyrivibrio fibrisolvens OB157 and OB189, respectively. Bfi57I (isoschizomer Sau3AI) had the DNA recognition/cleavage sequence 5'-/GATC-3'; it is not inhibited by Dam methylation, but is partially inhibited by M.BamHI methylation. Bfi89I (isoschizomer EaeI) had the recognition/cleavage sequence 5'-Y/GGCCR-3'; unlike the EaeI isoschizomer it is not fully inhibited by M.HaeIII methylation.
分别从溶纤维丁酸弧菌OB157和OB189中部分纯化出了两种II类限制性内切酶（ENases），即Bfi57I和Bfi89I。Bfi57I（同裂酶Sau3AI）具有DNA识别/切割序列5'-/GATC-3'；它不受Dam甲基化的抑制，但部分受M.BamHI甲基化的抑制。Bfi89I（同裂酶EaeI）具有识别/切割序列5'-Y/GGCCR-3'；与EaeI同裂酶不同，它不受M.HaeIII甲基化的完全抑制。
Zotero 插件