铜绿假单胞菌rpoH基因的克隆及一级序列
Cloning and primary sequence of the rpoH gene from Pseudomonas aeruginosa.
作者信息
Benvenisti L, Koby S, Rutman A, Giladi H, Yura T, Oppenheim A B
机构信息
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
出版信息
Gene. 1995 Mar 21;155(1):73-6. doi: 10.1016/0378-1119(94)00829-h.
PMID:7698670
Abstract
A DNA fragment from Pseudomonas aeruginosa containing the rpoH gene encoding the heat-shock sigma factor sigma 32 has been cloned and sequenced. The gene is expressed in Escherichia coli and complements an rpoH- strain. An open reading frame encoding 284 amino acids shows 61% identity and 78% similarity to the RpoH protein of Escherichia coli.
摘要
一段来自铜绿假单胞菌的包含编码热休克σ因子σ32的rpoH基因的DNA片段已被克隆并测序。该基因在大肠杆菌中表达，并能互补rpoH缺陷型菌株。一个编码284个氨基酸的开放阅读框与大肠杆菌的RpoH蛋白有61%的同一性和78%的相似性。
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