Mazerolles C, Rishmann P, Chopin D, Popov Z, Malavaud B, Selves J, Neulat-Duga I, Bellot J, Delsol G
Laboratoire d'anatomie pathologique, Centre d'Immunopathologie et de Génétique Humaine (CNRS), Clinique Centre Hospitalier Universitaire de Purpan, Toulouse, France.
Histopathology. 1994 Dec;25(6):563-8. doi: 10.1111/j.1365-2559.1994.tb01375.x.
The reactivity of MIB1 antibody on routinely processed paraffin sections was compared with that of Ki-67 antibody on frozen sections of 80 transitional cell carcinomas of the bladder. The percentage of labelled cells was expressed as the labelling index. MIB1 labelling indices were higher than those of Ki-67 but for each case the two values were strongly correlated (r = 0.91). Ki-67 and MIB1 indices were also correlated to tumour grade and stage (P < or = 0.001). MIB1 indices determined after both formaldehyde and ethanol based Bouin's fluid fixatives did not show any significant difference. MIB1 antibody staining after microwave oven heating of tissue sections is a simple technique for assessing the proliferative fraction of bladder tumours on fixed material. The use of MIB1 antibody permits retrospective studies and should determine whether the proliferation index in bladder carcinoma has the same prognostic value as demonstrated in other types of neoplasms.
将MIB1抗体在常规处理石蜡切片上的反应性与Ki-67抗体在80例膀胱移行细胞癌冰冻切片上的反应性进行比较。标记细胞的百分比表示为标记指数。MIB1标记指数高于Ki-67，但对于每例病例，这两个值呈强相关（r = 0.91）。Ki-67和MIB1指数也与肿瘤分级和分期相关（P≤0.001）。甲醛和乙醇基Bouin氏液固定后测定的MIB1指数无显著差异。组织切片经微波炉加热后的MIB1抗体染色是一种评估固定材料上膀胱肿瘤增殖分数的简单技术。使用MIB1抗体可进行回顾性研究，并应确定膀胱癌中的增殖指数是否具有与其他类型肿瘤中所证实的相同的预后价值。
