Bozlu Murat, Orhan Diclehan, Baltaci Sümer, Yaman Onder, Elhan Atilla Halil, Tulunay Ozden, Müftüoğlu Yusuf Ziya
Medical Faculty of Mersin University, Department of Urology, Turkey.
Int Urol Nephrol. 2002;33(1):59-66. doi: 10.1023/a:1014452902976.
To investigate the value of proliferating cell nuclear antigen (PCNA), Ki-67 antigen labelling indices and nucleolar organizer region (NOR) score in relation to histological grade, stage, recurrence and progression of the bladder tumor.
Tissue specimens from 77 bladder cancer patients (43 superficial, 34 invasive) were immunostained with PCNA and Ki-67 and stained with AgNOR. Thirteen specimens of normal bladder mucosa served as controls.
In comparison to normal bladder mucosa the values of the three indicators were significantly greater (p < 0.001). There was a significant relationship between PCNA, Ki-67 indices, AgNOR scores and grade and stage of the tumor (p < 0.001). All indicators also correlated with each other (p < 0.001). The Kaplan-Meier curves for recurrence-progression free survival revealed that patients with a PCNA labelling index >36.22%, Ki-67 labelling index >29.68% and AgNOR score > 3.34 had a worse prognosis than those with <36.22%, <29.68% and <3.34, respectively.
PCNA, Ki-67 indices and AgNOR scores correlated with each other and with tumor grade and stage. These proliferation markers may give objective and accurate information about the biological behavior of bladder transitional cell carcinoma.
研究增殖细胞核抗原（PCNA）、Ki-67抗原标记指数和核仁组织区（NOR）评分与膀胱肿瘤组织学分级、分期、复发及进展的关系。
对77例膀胱癌患者（43例表浅型，34例浸润型）的组织标本进行PCNA和Ki-67免疫染色及AgNOR染色。13例正常膀胱黏膜标本作为对照。
与正常膀胱黏膜相比，这三项指标的值均显著更高（p < 0.001）。PCNA、Ki-67指数、AgNOR评分与肿瘤分级和分期之间存在显著相关性（p < 0.001）。所有指标之间也相互关联（p < 0.001）。无复发进展生存期的Kaplan-Meier曲线显示，PCNA标记指数>36.22%、Ki-67标记指数>29.68%和AgNOR评分>3.34的患者预后分别比<36.22%、<29.68%和<3.34的患者差。
PCNA、Ki-67指数和AgNOR评分相互关联，且与肿瘤分级和分期相关。这些增殖标志物可能为膀胱移行细胞癌的生物学行为提供客观准确的信息。
