Gautam M, Mudd J, Copeland N G, Gilbert D J, Jenkins N A, Merlie J P
Department of Molecular Biology and Pharmacology, Washington University Medical School, St. Louis, Missouri 63110.
Genomics. 1994 Nov 15;24(2):366-9. doi: 10.1006/geno.1994.1629.
We have cloned and characterized mouse genomic DNA containing the gene for the 43-kDa acetylcholine receptor-associated protein. The gene extends over 12 kb and consists of 8 exons. RNase protection and sequence analysis have been used to define the intron/exon boundaries including 174 and 214 bp of 5' and 3' untranslated sequence in exons 1 and 8, respectively. Interestingly, the exon/intron organization is consistent with structural domains predicted from amino acid sequence conservation among 3 species of 43K. Finally, the 43K locus, designated Rapsn, has been mapped to the central region of mouse chromosome 2.
我们已经克隆并鉴定了包含43 kDa乙酰胆碱受体相关蛋白基因的小鼠基因组DNA。该基因跨度超过12 kb，由8个外显子组成。已使用核糖核酸酶保护和序列分析来确定内含子/外显子边界，其中第1外显子和第8外显子分别包含174 bp和214 bp的5'和3'非翻译序列。有趣的是，外显子/内含子组织与从3种43K物种之间的氨基酸序列保守性预测的结构域一致。最后，将命名为Rapsn的43K基因座定位到小鼠染色体2的中央区域。
