昆虫虹彩病毒6型基因组在基因组坐标0.310至0.347（7990碱基对）之间的一级结构和编码能力的鉴定。

Identification of the primary structure and the coding capacity of the genome of insect iridescent virus type 6 between the genome coordinates 0.310 and 0.347 (7990 bp).

作者信息

Sonntag K C, Schnitzler P, Janssen W, Darai G

机构信息

Institut für Medizinische Virologie, Universität Heidelberg, Germany.

出版信息

Intervirology. 1994;37(5):287-97. doi: 10.1159/000150390.

DOI:10.1159/000150390

PMID:7698884

Abstract

The primary structure and the coding capacity of the insect iridescent virus type 6--Chilo iridescent virus (CIV)--were determined between the genome coordinates 0.310 (EcoRI site) and 0.347 (ClaI site). The EcoRI CIV DNA fragment M (7.1 kb; 0.310-0.345 map units) harbors one out of at least six loci of DNA replication origins which is located at nucleotide position 485-513. The identification of the structural properties and the coding capacity of the EcoRI CIV DNA fragment M was carried out by DNA nucleotide sequencing, computer-aided sequence analysis and DNA/RNA hybridization. The EcoRI CIV DNA fragment M (7,099 bp; 71.14% A+T and 28.86% G+C) possesses two clusters of five tandemly organized repetitive DNA elements with complex structural arrangements (R1-R5) which are located between nucleotide positions 3272-3350 and 3403-3414. The analysis of the DNA sequences of the EcoRI CIV DNA fragment M revealed the presence of six open reading frames (ORFs 1-6). Two out of six detected putative proteins are of particular interest. ORF-2 was found to be terminated at nucleotide position 366 (TAA) within the DNA sequence of the EcoRI CIV DNA fragment L (0.345-0.381 map units; 7.4 kb). The analysis of ORF-2 (1,051 amino acids; 120 kD) revealed homologies to several DNA-directed RNA polymerases. ORF-6 encodes a protein (606 amino acids; 69 kD) which is related to a group of yeast, Drosophila and mammalian proteins of a distinct family of putative DNA and/or RNA helicases belonging to the 'DEAD/H' superfamily. The transcriptional activity of the EcoRI CIV DNA fragment M was determined by DNA/RNA hybridization experiments. These analyses revealed the existence of three RNA transcripts of about 3.4 kb (t1), 1.8 kb (t2) and 1.2 kb (t3) which agree with the predicted size of the expected RNA transcripts from ORF-2 (1,051 amino acids; 3.1 kb) and ORF-6 (606 amino acids; 1.8 kb).

摘要

在基因组坐标0.310（EcoRI位点）和0.347（ClaI位点）之间确定了昆虫虹彩病毒6型——稻纵卷叶螟虹彩病毒（CIV）的一级结构和编码能力。EcoRI CIV DNA片段M（7.1 kb；0.310 - 0.345图距单位）含有至少六个DNA复制起始位点中的一个，该位点位于核苷酸位置485 - 513处。通过DNA核苷酸测序、计算机辅助序列分析和DNA/RNA杂交对EcoRI CIV DNA片段M的结构特性和编码能力进行了鉴定。EcoRI CIV DNA片段M（7099 bp；71.14% A + T和28.86% G + C）拥有两组由五个串联组织的重复DNA元件组成的簇，其结构排列复杂（R1 - R5），位于核苷酸位置3272 - 3350和3403 - 3414之间。对EcoRI CIV DNA片段M的DNA序列分析揭示了六个开放阅读框（ORF 1 - 6）的存在。六个检测到的推定蛋白中有两个特别令人感兴趣。发现ORF - 2在EcoRI CIV DNA片段L（0.345 - 0.381图距单位；7.4 kb）的DNA序列内的核苷酸位置366（TAA）处终止。对ORF - 2（1051个氨基酸；120 kD）的分析揭示了它与几种DNA指导的RNA聚合酶的同源性。ORF - 6编码一种蛋白（606个氨基酸；69 kD），该蛋白与酵母、果蝇和哺乳动物中属于“DEAD/H”超家族的一个独特的推定DNA和/或RNA解旋酶家族的蛋白相关。通过DNA/RNA杂交实验确定了EcoRI CIV DNA片段M的转录活性。这些分析揭示了存在三种约3.4 kb（t1）、1.8 kb（t2）和1.2 kb（t3）的RNA转录本，它们与来自ORF - 2（1051个氨基酸；3.1 kb）和ORF - 6（606个氨基酸；1.8 kb）的预期RNA转录本的预测大小一致。