利用酵母杀伤系统鉴定星形诺卡菌复合体的菌种。
Use of yeast killer system to identify species of the Nocardia asteroides complex.
作者信息
Provost F, Polonelli L, Conti S, Fisicaro P, Gerloni M, Boiron P
机构信息
Unité de Mycologie, Institut Pasteur, Paris, France.
出版信息
J Clin Microbiol. 1995 Jan;33(1):8-10. doi: 10.1128/jcm.33.1.8-10.1995.
We evaluated the ability of the yeast killer system to differentiate members belonging to the Nocardia asteroides complex (Nocardia asteroides, Nocardia farcinica, and Nocardia nova). Nocardia strains were selected randomly from clinical isolates. Type strains of each Nocardia species and recognized killer yeasts were taken from different collections. A clear area of inhibition surrounding the yeast cells demonstrated a positive killer effect on Nocardia spp. Two yeast strains, Pichia mrakii (K9) and Pichia lynferdii (K76), showed different killer activities against each Nocardia species. The group N. asteroides was identified as K9+ K76-, the group N. farcinica was identified as K9- K76-, and the group N. nova was identified as K9+ K76+. The three killer identifications correlated with specific taxonomic groups determined by using classical methods. The yeast killer system may be a useful means for identifying organisms within the N. asteroides complex.
我们评估了酵母杀伤系统区分属于星形诺卡菌复合体（星形诺卡菌、豚鼠耳炎诺卡菌和新星诺卡菌）成员的能力。诺卡菌菌株是从临床分离株中随机选取的。每种诺卡菌的模式菌株和公认的杀伤酵母取自不同的菌种保藏库。酵母细胞周围出现清晰的抑菌圈表明对诺卡菌属有阳性杀伤作用。两株酵母，马克毕赤酵母（K9）和林氏毕赤酵母（K76），对每种诺卡菌表现出不同的杀伤活性。星形诺卡菌群被鉴定为K9 + K76 -，豚鼠耳炎诺卡菌群被鉴定为K9 - K76 -，新星诺卡菌群被鉴定为K9 + K76 +。这三种杀伤鉴定结果与用经典方法确定的特定分类群相关。酵母杀伤系统可能是鉴定星形诺卡菌复合体内微生物的一种有用手段。
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