核糖体分型：用于星形诺卡菌复合体物种分类和鉴定的工具
Ribotyping: a tool for taxonomy and identification of the Nocardia asteroides complex species.
作者信息
Laurent F, Carlotti A, Boiron P, Villard J, Freney J
机构信息
Department de Recherche en Bacteriologie (E.A. 1655), Faculté de Médecine A. Carrel, Lyon, France.
出版信息
J Clin Microbiol. 1996 May;34(5):1079-82. doi: 10.1128/jcm.34.5.1079-1082.1996.
Abstract
Ribotyping has been evaluated as a taxonomic tool for studying bacteria belonging to the Nocardia asteroides complex. The probe used was obtained by PCR from DNA extracted from the type strain Nocardia asteroides ATCC 19247, a sequence that codes partly for 16S rRNA. Interpretation of hybridization after EcoRI restriction of total DNA of 21 strains of the N. asteroides complex allowed for the identification of only four different ribotypes: two related to N. asteroides sensu stricto, one related to N. farcinica and one related to N. nova. These results confirm the classification established by Tsukamura in 1982 (M. Tsukamura, Microbiol. Immunol. 26:1101-1119, 1982) as well as the heterogeneity of N. asteroides sensu stricto species. Ribotyping may also provide a major identification technique for the species belonging to the N. asteroides complex.
摘要
核糖体分型已被评估为一种用于研究属于星形诺卡菌复合体细菌的分类工具。所使用的探针是通过PCR从模式菌株星形诺卡菌ATCC 19247提取的DNA中获得的，该序列部分编码16S rRNA。对21株星形诺卡菌复合体菌株的总DNA进行EcoRI酶切后的杂交结果分析，仅鉴定出四种不同的核糖体类型：两种与狭义星形诺卡菌相关，一种与豚鼠耳炎诺卡菌相关，一种与新星诺卡菌相关。这些结果证实了冢村于1982年建立的分类（M. 冢村，《微生物学与免疫学》26:1101 - 1119, 1982）以及狭义星形诺卡菌物种的异质性。核糖体分型也可能为属于星形诺卡菌复合体的物种提供一种主要的鉴定技术。
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