Cunningham K D, Cecava M J, Johnson T R
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907.
J Dairy Sci. 1994 Dec;77(12):3666-75. doi: 10.3168/jds.S0022-0302(94)77311-2.
Four Holstein cows, fitted with ruminal and duodenal cannulas, were used to determine the effects of supplemental feather meal and blood meal on ruminal fermentation and flows of N and AA to the duodenum. The basal diet contained (DM basis) 9.9% chopped alfalfa hay, 39.7% corn silage, 34.7% cracked corn, 8.2% corn starch, 2.1% vitamins and minerals, 4.4% casein, and 1% urea. A combination of feather meal and blood meal (3:1 on an N basis) was used to replace 0, 33, 67, and 100% of the casein and urea in the basal diet in a Latin square design. Intakes of DM, OM, and N were similar for all diets. Flows of total N, NAN, individual AA, total AA, and total essential AA were unaltered as supplemental feather meal plus blood meal increased. Flows of microbial N tended to decrease, but flow of non-ammonia, nonmicrobial N increased as supplemental feather meal plus blood meal increased. Increased proportions of dietary feather plus blood meal decreased the proportions of Ile, Lys, Met, and Thr in duodenal digesta. Molar percentages of ruminal pH and VFA were unchanged, but concentrations of ruminal NH3 N decreased linearly as supplemental feather meal plus blood meal increased. For the diets fed in this study, inclusion of one-third of the supplemental protein from feather plus blood meal resulted in maximum flows of NAN and microbial N to the small intestine.
选用4头安装了瘤胃和十二指肠瘘管的荷斯坦奶牛,以确定补充羽毛粉和血粉对瘤胃发酵以及氮和氨基酸向十二指肠流动的影响。基础日粮(以干物质计)包含9.9%的切碎苜蓿干草、39.7%的玉米青贮、34.7%的碎玉米、8.2%的玉米淀粉、2.1%的维生素和矿物质、4.4%的酪蛋白以及1%的尿素。采用拉丁方设计,用羽毛粉和血粉的混合物(以氮计为3:1)替代基础日粮中0、33、67和100%的酪蛋白和尿素。所有日粮的干物质、有机物和氮摄入量相似。随着补充羽毛粉加血浆粉量的增加,总氮、非氨氮、单个氨基酸、总氨基酸和总必需氨基酸的流量未发生改变。微生物氮流量有下降趋势,但随着补充羽毛粉加血浆粉量的增加,非氨、非微生物氮流量增加。日粮中羽毛粉加血浆粉比例的增加降低了十二指肠消化物中异亮氨酸、赖氨酸、蛋氨酸和苏氨酸的比例。瘤胃pH值和挥发性脂肪酸的摩尔百分比未发生变化,但随着补充羽毛粉加血浆粉量的增加,瘤胃氨氮浓度呈线性下降。对于本研究中所饲喂的日粮,从羽毛粉加血浆粉中补充三分之一的蛋白质可使非氨氮和微生物氮向小肠的流量达到最大。