Halpern Z, Lafont H, Arad J, Domingo N, Peled Y, Konikoff F, Gilat T
Department of Gastroenterology, Tel Aviv Medical Center, Israel.
J Hepatol. 1994 Dec;21(6):979-83. doi: 10.1016/s0168-8278(05)80605-1.
The small (7 kD) biliary phospholipid and calcium binding polypeptide (anionic polypeptide fraction/calcium binding protein) has been found in higher concentrations in the bile of patients with pigment stones than in controls. In different model systems it was variously found to promote or retard cholesteral crystalization. In the present study we investigated its distribution between cholesterol carriers in bile and its effect on cholesterol crystalization in native and model biles. On gel chromatography anionic polypeptide fraction/calcium binding protein was found predominantly in three areas: in the vesicular fraction, in the non-vesicular lipid fraction and in another fraction unassociated with biliary lipids. It was much more concentrated in the vesicular than in the non-vesicular fraction, the mean anionic polypeptide fraction/phospholipid molar ratio being 219 +/- 181 vs. 30.4 +/- 16, respectively. Anionic polypeptide fraction/calcium binding protein was added at three dose levels, 0.14, 0.28, 0.42 mg/ml (representing approximately 18%-55% of the physiologic biliary concentration), to 19 human and five model biles. This did not produce any significant changes in the nucleation time. The addition of anionic polypeptide fraction/calcium binding protein at a dose level of 0.42 mg/ml to 13 different human biles did not induce changes in the distribution of cholesterol among its carriers. The present experiments do not support a role for anionic polypeptide fraction/calcium binding protein in the process of cholesterol nucleation in bile. Qualitative changes in the protein molecule, as demonstrated in other human secretions, cannot be excluded.
已发现小分子量（7kD）的胆汁磷脂和钙结合多肽（阴离子多肽组分/钙结合蛋白）在色素结石患者胆汁中的浓度高于对照组。在不同的模型系统中，发现它对胆固醇结晶有促进或抑制作用。在本研究中，我们研究了它在胆汁中胆固醇载体之间的分布及其对天然胆汁和模型胆汁中胆固醇结晶的影响。凝胶色谱分析显示，阴离子多肽组分/钙结合蛋白主要分布在三个区域：囊泡组分、非囊泡脂质组分以及另一个与胆汁脂质无关的组分中。它在囊泡组分中的浓度远高于非囊泡组分，阴离子多肽组分与磷脂的平均摩尔比分别为219±181和30.4±16。将阴离子多肽组分/钙结合蛋白以0.14、0.28、0.42mg/ml三个剂量水平（分别约为生理胆汁浓度的18%-55%）添加到19份人胆汁和5份模型胆汁中。这并未使成核时间发生任何显著变化。将0.42mg/ml剂量水平的阴离子多肽组分/钙结合蛋白添加到13份不同的人胆汁中，并未引起胆固醇在其载体间分布的改变。本实验不支持阴离子多肽组分/钙结合蛋白在胆汁中胆固醇成核过程中发挥作用。但不能排除该蛋白分子如在其他人体分泌物中所显示的那样发生定性变化的可能性。
