Wakayama Y, Jimi T, Takeda A, Shibuya S, Nakamura Y, Oniki H
Department of Medicine, Showa University Fujigaoka Hospital, Yokohama, Japan.
J Electron Microsc (Tokyo). 1994 Oct;43(5):327-31.
Ultrastructural localization of the C-terminus of a 43-kDa dystrophin-associated glycoprotein (43-DAG) and its relation to dystrophin were investigated in normal human skeletal myofibers by using single and double immunogold labelling methods. The C-terminal end of 43-DAG antibody was localized ultrastructurally at the inside surface of muscle plasma membrane and the sarcoplasmic side of the plasma membrane invaginations. Double immunolabelling electron microscopy disclosed that signals of the C-terminal end of 43-DAG and dystrophin were often associated with each other and formed a doublet at the inside surface of plasma membranes in normal human skeletal myofibers.
采用单免疫金标记和双免疫金标记方法，在正常人骨骼肌肌纤维中研究了43 kDa抗肌萎缩蛋白相关糖蛋白（43-DAG）C末端的超微结构定位及其与抗肌萎缩蛋白的关系。43-DAG抗体的C末端在超微结构上定位于肌细胞膜的内表面以及细胞膜内陷的肌浆侧。双重免疫标记电子显微镜显示，在正常人骨骼肌肌纤维中，43-DAG C末端和抗肌萎缩蛋白的信号常常相互关联，并在细胞膜内表面形成双联体。
