Jones J V, Eastwood B J, Jones E, James H, Mansour M
Department of Medicine, Dalhousie University, Halifax, NS, Canada.
J Rheumatol. 1995 Jan;22(1):55-61.
To determine the distribution of antibodies to cardiolipin (IgG, IgM and IgA) in a healthy population. To classify subjects according to a cutpoint in order to determine what level of antibody measurement would yield 95% specificity.
Antiphospholipid (aPL) antibodies were measured using a conventional ELISA technique, with isotype specific antibody, to determine levels of IgG, IgM and IgA binding to cardiolipin. The subjects were 282 Red Cross blood donors, 82 undergraduate medical and nursing students, and 13 laboratory volunteers.
The distribution of aPL antibodies measured in units of optical density is significantly skewed, while the distribution of aPL antibodies measured on the log scale is nearly symmetric. Neither distribution is normally distributed. There was no evidence that aPL antibodies differed significantly by age (up to age 50) or sex. We estimate the 90th, 95th, and 99th percentiles for IgG, IgM and IgA antibodies for the optical densities and binding indices.
For 95% specificity we conclude that an adjusted IgG optical density above 0.386, an adjusted IgM optical density above 0.301, or an adjusted IgA optical density above 0.085 be considered positive.
确定健康人群中抗心磷脂抗体（IgG、IgM和IgA）的分布情况。根据一个切点对受试者进行分类，以确定何种抗体测量水平可产生95%的特异性。
采用传统酶联免疫吸附测定（ELISA）技术，使用同型特异性抗体，测定与心磷脂结合的IgG、IgM和IgA水平。受试者包括282名红十字会献血者、82名医学和护理专业本科生以及13名实验室志愿者。
以光密度单位测量的抗磷脂（aPL）抗体分布明显偏态，而以对数尺度测量的aPL抗体分布近乎对称。两种分布均不呈正态分布。没有证据表明aPL抗体在年龄（50岁及以下）或性别上存在显著差异。我们估计了IgG、IgM和IgA抗体光密度和结合指数的第90、95和99百分位数。
对于95%的特异性，我们得出结论，调整后的IgG光密度高于0.386、调整后的IgM光密度高于0.301或调整后的IgA光密度高于0.085可被视为阳性。
