一种突变型1型血管紧张素II受体的特性分析。

Characterization of a mutant type-1 angiotensin II receptor.

作者信息

Roche P J, Eddie L W, Coghlan J P

机构信息

Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Australia.

出版信息

Kidney Int. 1994 Dec;46(6):1580-2. doi: 10.1038/ki.1994.452.

DOI:10.1038/ki.1994.452

PMID:7700012

Abstract

We have used PCR to amplify the rat AT1 receptor from liver mRNA. The receptor DNA was subcloned into pcDNAI and a number of individual clones were transiently expressed in COS-7 cells. These individual receptors were characterized by binding of [125I] Sar1, Ile8-angiotensin II. It was found that one of the AT1 receptor clones did not bind the radiolabeled angiotensin II ligand. The mutant clone was analyzed by nucleotide sequencing. This analysis revealed that the clone has a single amino acid substitution in the third transmembrane domain of the receptor; a leucine at position 112 was changed to a proline. At this stage it is not known whether the mutant receptor is the result of a PCR artifact or an allelic difference.

摘要

我们利用聚合酶链反应（PCR）从大鼠肝脏信使核糖核酸（mRNA）中扩增出血管紧张素Ⅱ1型（AT1）受体。将该受体DNA亚克隆到质粒pcDNAI中，并使多个单个克隆在COS - 7细胞中瞬时表达。通过[125I]Sar1、Ile8 - 血管紧张素Ⅱ的结合对这些单个受体进行表征。结果发现，其中一个AT1受体克隆不与放射性标记的血管紧张素Ⅱ配体结合。通过核苷酸测序对该突变克隆进行分析。该分析表明，该克隆在受体的第三个跨膜结构域有一个单氨基酸取代；第112位的亮氨酸变为脯氨酸。目前尚不清楚该突变受体是聚合酶链反应假象的结果还是等位基因差异。