Gergen K A, Chang S L, Niu Y F, Kastin A J, Zadina J E
VA Medical Center, New Orleans, LA 70146.
Peptides. 1994;15(8):1505-11. doi: 10.1016/0196-9781(94)90130-9.
Tyr-W-MIF-1 is a tetrapeptide recently isolated from brain that has opiate modulating activity. In this study, we used immunocytochemical (ICC) detection of FOS proto-oncogene protein to map brain areas activated by an ICV injection of Tyr-W-MIF-1 (200 micrograms). The analgesic effect of the peptide, which lasted 1 h, was confirmed in each rat with the tail flick test. FOS was activated in several limbic structures, including the cingulate and infralimbic cortex, nucleus accumbens, and central nucleus of the amygdala. FOS activation also occurred in several diencephalic nuclei, including the supraoptic, paraventricular, and periventricular nuclei of the hypothalamus, and the paraventricular nucleus of the thalamus. Several activated areas contained mu-opiate receptors. However, despite the known selectivity of Tyr-W-MIF-1 for mu receptors, FOS immunoreactivity was also induced in nuclei of the amygdala, hypothalamus, and thalamus, where concentrations of kappa receptors were high but those of mu and delta receptors were not detected. The results show that Tyr-W-MIF-1 induces FOS activation in several brain areas, including but not limited to, areas associated with nociception and stress-induced analgesia.
酪氨酰 - 色氨酸 - 甲硫氨酸 - 异亮氨酸 - 苯丙氨酸 - 1（Tyr-W-MIF-1）是一种最近从大脑中分离出来的具有阿片类调节活性的四肽。在本研究中，我们使用免疫细胞化学（ICC）检测FOS原癌基因蛋白，以绘制经脑室内注射Tyr-W-MIF-1（200微克）激活的脑区图谱。通过甩尾试验在每只大鼠中证实了该肽持续1小时的镇痛作用。FOS在几个边缘结构中被激活，包括扣带回和边缘下皮质、伏隔核以及杏仁核中央核。FOS激活也发生在几个间脑核中，包括下丘脑的视上核、室旁核和室周核以及丘脑的室旁核。几个激活区域含有μ-阿片受体。然而，尽管已知Tyr-W-MIF-1对μ受体具有选择性，但在杏仁核、下丘脑和丘脑的核中也诱导了FOS免疫反应性，其中κ受体浓度高，但未检测到μ和δ受体浓度。结果表明，Tyr-W-MIF-1在几个脑区诱导FOS激活，包括但不限于与伤害感受和应激诱导镇痛相关的区域。
