Savchenkova I P, Sergeev N I, Aĭtbaev N E
Tsitologiia. 1994;36(9-10):955-9.
Conditions for isolation of pluripotent inner cell masses (ICM) from rabbit blastocysts are described. The rabbit intact embryos were collected on day 4 post coitus, zona pellucida was removed from embryos by pronase treatment, and rabbit blastocysts were plated on different monolayers of somatic cells. The differences in attachment and proliferation of ICM of rabbit embryos were determined in dependence on the feeder layer type on their long culturing in vitro. Among the tested monolayers of somatic cells a feeder layer of rabbit embryonic fibroblasts, derived from fetuses on day 16-18 pregnancy, appeared more effective for isolation of ICM and for receipt of rabbit embryonic stem cells (with ES-like morphology).
本文描述了从兔囊胚中分离多能内细胞团（ICM）的条件。在交配后第4天收集兔完整胚胎，用链霉蛋白酶处理去除胚胎的透明带，然后将兔囊胚接种到不同的体细胞单层上。根据体外长期培养时饲养层类型的不同，测定兔胚胎ICM在附着和增殖方面的差异。在所测试的体细胞单层中，源自妊娠第16 - 18天胎儿的兔胚胎成纤维细胞饲养层，在分离ICM和获得兔胚胎干细胞（具有类ES细胞形态）方面似乎更有效。
