流式细胞术：免疫血液学中用于评估D+W（Du）抗原的工具？

Flow cytometry: a tool in immunohematology for D+W (Du) antigen evaluation?

作者信息

Tazzari P L, Bontadini A, Belletti D, Malferrari F, Conte R

机构信息

Servizio di Immunoematologia, Policlinico S. Orsola, Bologna, Italy.

出版信息

Vox Sang. 1994;67(4):382-6. doi: 10.1111/j.1423-0410.1994.tb01278.x.

DOI:10.1111/j.1423-0410.1994.tb01278.x

PMID:7701810

Abstract

We performed a flow-cytometric analysis of the expression of D antigen in D+W samples (previously termed Du). We also analysed a series of D-positive and D-negative (cde phenotype) samples to obtain positive and negative controls, respectively. The evaluation was carried out by immunofluorescence and the intensity of positivity was expressed as mean channel value (MCV) of fluorescence. Results demonstrated that D+W samples have lower expression (less than 1 log) than D-positive cases (p > 0.001, Student t test), while cde samples show the same MCVs as negative controls. Moreover, it was also possible to set a grading of D antigen expression and to analyze cases difficult to assess by agglutination only.

摘要

我们对D+W样本（以前称为Du）中D抗原的表达进行了流式细胞术分析。我们还分析了一系列D阳性和D阴性（cde表型）样本，分别作为阳性和阴性对照。通过免疫荧光进行评估，阳性强度以荧光的平均通道值（MCV）表示。结果表明，D+W样本的表达低于D阳性病例（小于1个对数）（p>0.001，学生t检验），而cde样本的MCV与阴性对照相同。此外，还可以对D抗原表达进行分级，并分析仅通过凝集难以评估的病例。