Wibawan I W, Lämmler C
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, Germany.
Zentralbl Veterinarmed B. 1994 Oct;41(7-8):453-9. doi: 10.1111/j.1439-0450.1994.tb00250.x.
Encapsulation is thought to be a critical virulence factor for Streptococcus suis. In this study, encapsulation of a selected S. suis culture could be stimulated by cultivation conditions. After cultivation of the S. suis culture in fluid media supplemented with serum, the culture grew with short chains and a uniform turbidity of the growth medium. Autoclave extracts of this culture reacted with capsular type-1-specific antiserum. After cultivation of the S. suis culture in fluid media without serum, the bacteria generally formed longer chains, grew in fluid medium as granular sediment with clear supernatant, and were non-typeable. In addition, the unencapsulated variant appeared to have a more hydrophobic surface and adhered significantly more to HeLa cells. In contrast to the unencapsulated variant, the encapsulated S. suis was phagocytosed to a lesser extent by polymorphonuclear leucocytes. These findings might help to elucidate the role of encapsulation in infections with this bacterial organism.
荚膜形成被认为是猪链球菌的一个关键毒力因子。在本研究中，特定猪链球菌培养物的荚膜形成可受到培养条件的刺激。在添加血清的液体培养基中培养猪链球菌培养物后，培养物呈短链生长，生长培养基浊度均匀。该培养物的高压灭菌提取物与1型荚膜特异性抗血清发生反应。在无血清的液体培养基中培养猪链球菌培养物后，细菌通常形成更长的链，在液体培养基中呈颗粒状沉淀生长，上清液澄清，且无法分型。此外，无荚膜变体似乎具有更疏水的表面，并且对HeLa细胞的黏附明显更多。与无荚膜变体相比，有荚膜的猪链球菌被多形核白细胞吞噬的程度较小。这些发现可能有助于阐明荚膜形成在这种细菌感染中的作用。
