Stram Y, Meir R, Molad T, Blumenkranz R, Malkinson M, Weisman Y
Kimron Veterinary Institute, Bet-Dagan, Israel.
Avian Dis. 1994 Oct-Dec;38(4):879-84.
Reverse transcriptase-polymerase chain reaction was used for identification of Israeli isolates of infectious bursal disease virus (IBDV). The system was applied to tissue culture and to bursa of Fabricius from infected chickens; these latter samples had been frozen for as long as 4 years. From base homology analysis of published sequences of serotype 1 IBDV, two pairs of primers, targeted to amplify sequences from the VP2 and VP3 cistrons, were prepared. The two sets of primers could detect viruses of serotype 1. The primers directed to the cistrons could detect viral sequences from seven infected chickens. No reaction was detected with RNA extracted from bursal cells of healthy chickens or from uninfected cells. The sensitivity of the reaction was equivalent to 2.5 x 10(1) TCID50.
逆转录聚合酶链反应被用于鉴定传染性法氏囊病病毒（IBDV）的以色列分离株。该系统应用于组织培养以及来自感染鸡的法氏囊；后者的样本已冷冻长达4年。根据已发表的1型IBDV序列的碱基同源性分析，制备了两对引物，用于扩增VP2和VP3顺反子的序列。这两组引物能够检测1型病毒。针对顺反子的引物能够检测来自7只感染鸡的病毒序列。从健康鸡的法氏囊细胞或未感染细胞中提取的RNA未检测到反应。该反应的灵敏度相当于2.5×10(1) TCID50。
