Novak U, Paradiso L, Hamilton J A
University of Melbourne, Department of Medicine, Royal Melbourne Hospital, Parkville, Victoria, Australia.
DNA Cell Biol. 1994 Nov;13(11):1063-9. doi: 10.1089/dna.1994.13.1063.
The promoter of the human urokinase plasminogen activator (uPA) gene contains a sequence identical with the retinoblastoma control element (RCE) of the murine c-fos gene, as well as several Sp1 binding sites. In a number of cell lines, the uPA promoter is activated during enforced expression of the retinoblastoma protein, pRB. Electrophoretic mobility-shift assays revealed that the RCE sequence of the uPA gene forms only one specific DNA-protein complex that does not contain pRB. The formation of the RCE-protein complex can be inhibited by 20 molar excess of the unlabeled RCE sequences and by 5 molar excess of the unlabeled E2F binding site. The RCE of the human uPA gene interacts specifically with a protein, which appears to be distinct from members of the E2F family of proteins, Sp1, ATF2, and Elf-1, which are all transcription factors shown to be regulated by pRB.
人尿激酶型纤溶酶原激活剂（uPA）基因的启动子包含一段与鼠c-fos基因的视网膜母细胞瘤控制元件（RCE）相同的序列，以及多个Sp1结合位点。在许多细胞系中，uPA启动子在视网膜母细胞瘤蛋白pRB的强制表达过程中被激活。电泳迁移率变动分析显示，uPA基因的RCE序列仅形成一种不包含pRB的特异性DNA-蛋白质复合物。RCE-蛋白质复合物的形成可被20摩尔过量的未标记RCE序列和5摩尔过量的未标记E2F结合位点所抑制。人uPA基因的RCE与一种蛋白质特异性相互作用，该蛋白质似乎不同于E2F蛋白家族的成员、Sp1、ATF2和Elf-1，而这些都是已证明受pRB调控的转录因子。
