兔精子蛋白Sp17在COS细胞中的表达及重组Sp17与兔透明带的相互作用。
Expression of the rabbit sperm protein Sp17 in COS cells and interaction of recombinant Sp17 with the rabbit zona pellucida.
作者信息
Yamasaki N, Richardson R T, O'Rand M G
机构信息
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
出版信息
Mol Reprod Dev. 1995 Jan;40(1):48-55. doi: 10.1002/mrd.1080400107.
PMID:7702869
Abstract
This study extends our analysis of rabbit recombinant Sp17 (rSp17) by examining whether rSp17 synthesized in transfected COS cells will show a particular localization within the cell and whether the COS cell will bind with zona pellucida. We show, using the crosslinking, reagent DSS that rSp17 can bind to rabbit zona glycoprotein R45 or R55.
摘要
本研究通过检测转染的COS细胞中合成的兔重组Sp17（rSp17）是否会在细胞内呈现特定定位，以及COS细胞是否会与透明带结合，扩展了我们对rSp17的分析。我们使用交联剂二硫代双琥珀酰亚胺酯（DSS）表明，rSp17能够与兔透明带糖蛋白R45或R55结合。
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