Rosenthal A L, Igdoura S A, Morales C R, Hermo L
Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada.
Mol Reprod Dev. 1995 Jan;40(1):69-83. doi: 10.1002/mrd.1080400110.
The objective of this study was to define the factors regulating the endogenous production of sulfated glycoprotein-1 (SGP-1) in nonciliated cells of the efferent ducts. To this end we examined five different groups of animals undergoing the following experimental procedures: (1) hypophysectomized animals at 7, 14, and 28 days, (2) 7-day hypophysectomized rats receiving testosterone implants given at various time intervals thereafter, (3) castration at various time intervals up to 7 days, (4) 7-day castrated rats receiving testosterone implants at various time intervals thereafter, and (5) castrated rats given testosterone implants immediately after castration and sacrificed at different time intervals thereafter. Efferent ducts were fixed by perfusion with 4% paraformaldehyde and 0.5% glutaraldehyde in phosphate buffer for quantitative immunocytochemical analysis at the level of the electron microscope. For each experimental condition and their controls, the number of gold particles/micron2 within the endosomal and lysosomal compartments was calculated taking into account the changes in both the volume of the cell and organelles being quantified and expressed as labeling content. The results revealed that hypophysectomy (up to 4 weeks) caused a marked significant decrease in the SGP-1 labeling content of the endosomal and lysosomal compartments. The labeling content of the lysosomal compartment of efferent ducts from rats castrated for up to 1 week did not change significantly. However, there was a significant decrease in the labeling content of endosomes. This decrease is due to SGP-1, which is secreted by Sertoli cells, not being available for uptake in the efferent ducts. These results suggested that testosterone is not required for maintaining the high labeling content of SGP-1 within lysosomes of nonciliated cells, but that a pituitary factor appears to be needed. The administration of testosterone at different intervals to 7-day castrated animals resulted in a significant decrease of lysosomal SGP-1, suggesting that testosterone under these experimental conditions inhibits the production of a pituitary factor that maintains the high labeling content of SGP-1 within lysosomes of the nonciliated cells. Testosterone administered to 7-day hypophysectomized animals over a 24-hr period had no effect on the labeling content of SGP-1 within lysosomes. However, the administration of testosterone to animals immediately following castration showed no differences in the labeling content of SGP-1 within compared to controls.(ABSTRACT TRUNCATED AT 400 WORDS)
本研究的目的是确定调节输出小管非纤毛细胞中硫酸化糖蛋白-1（SGP-1）内源性产生的因素。为此，我们检查了五组接受以下实验程序的动物：（1）术后7天、14天和28天的垂体切除动物；（2）术后7天垂体切除的大鼠，此后在不同时间间隔植入睾酮；（3）在长达7天的不同时间间隔进行阉割；（4）术后7天阉割的大鼠，此后在不同时间间隔植入睾酮；（5）阉割后立即植入睾酮并在之后不同时间间隔处死的大鼠。通过在磷酸盐缓冲液中用4%多聚甲醛和0.5%戊二醛灌注固定输出小管，以便在电子显微镜水平进行定量免疫细胞化学分析。对于每种实验条件及其对照，计算内体和溶酶体区室内每平方微米的金颗粒数量，同时考虑到所量化的细胞和细胞器体积的变化，并将其表示为标记含量。结果显示，垂体切除（长达4周）导致内体和溶酶体区室的SGP-1标记含量显著明显降低。长达1周阉割的大鼠输出小管溶酶体区室的标记含量没有显著变化。然而，内体的标记含量显著降低。这种降低是由于支持细胞分泌的SGP-1无法在输出小管中被摄取。这些结果表明，维持非纤毛细胞溶酶体内SGP-1的高标记含量不需要睾酮，但似乎需要一种垂体因子。在术后7天阉割的动物中不同时间间隔给予睾酮导致溶酶体SGP-1显著降低，表明在这些实验条件下睾酮抑制了维持非纤毛细胞溶酶体内SGP-1高标记含量的垂体因子的产生。在24小时内给予术后7天垂体切除的动物睾酮对溶酶体内SGP-1的标记含量没有影响。然而，阉割后立即给予动物睾酮，与对照组相比，溶酶体内SGP-1的标记含量没有差异。（摘要截选至400字）
