Thomas K L, Davis S, Laroche S, Hunt S P
Neurobiology Division, Medical Research Council Centre, Cambridge, UK.
Neuroreport. 1994 Dec 30;6(1):119-23. doi: 10.1097/00001756-199412300-00031.
Several isoforms of the NMDA receptor 1 (NR1) subunits of the ionotropic NMDA (N-methyl-D-aspartate) glutamate receptor contain a consensus site for phosphorylation by protein kinase C (PKC) which, once phosphorylated results in an increased conductance through the receptor channel. Using in situ hybridization, we investigated the expression of NR1 subunits sensitive or insensitive to modification by PKC in the granule cells of the dentate gyrus of the hippocampus, following the induction of long-term potentiation (LTP). A selective 50% increase only in the levels of mRNA NR1 subunits containing this consensus sequence for PKC phosphorylation was seen 48 h after LTP induction. The change in the expression of PKC-sensitive NR1 subunits may be the molecular basis for the increased response of the post-synaptic cell to released glutamate during the maintenance phase of LTP.
离子型N-甲基-D-天冬氨酸（NMDA）谷氨酸受体的NMDA受体1（NR1）亚基的几种同工型含有蛋白激酶C（PKC）磷酸化的共有位点，一旦被磷酸化，会导致通过受体通道的电导增加。利用原位杂交技术，我们研究了在海马齿状回颗粒细胞中，诱导长时程增强（LTP）后，对PKC修饰敏感或不敏感的NR1亚基的表达情况。在LTP诱导后48小时，仅观察到含有该PKC磷酸化共有序列的mRNA NR1亚基水平选择性地增加了50%。PKC敏感的NR1亚基表达的变化可能是LTP维持阶段突触后细胞对释放的谷氨酸反应增强的分子基础。
