Rivard C H, Chaput C J, DesRosiers E A, Yahia L H, Selmani A
Department of Orthopaedic Surgery, Hôpital Sainte-Justine, Montréal, Canada.
J Appl Biomater. 1995 Spring;6(1):65-8. doi: 10.1002/jab.770060109.
A natural poly(hydroxybutyrate-co-9% hydroxyvalerate) copolyester was processed into a three-dimensional porous foam structure by salt leaching/solvent casting with previously sieved sodium chloride salts. Laboratory-built P(HB-9% HV) foams and commercial collagen sponges were cut into small rectangular specimens, sterilized, and prewetted using ethanol, rinsed with Dulbecco's minimum essential medium + 10% serum culture media, and seeded with fibroblasts isolated from canine anterior cruciate ligaments. The fibroblast cultures into such porous substrates were performed from 0 to 35 days by incubation (5% CO2) at 37 degrees C. It demonstrated that the P(HB-HV) sustained a cell proliferation rate similar to that observed in collagen sponges, up to at least 35 days, with a maximal cell density on the day 28 in culture. On the other hand, the P(HB-HV) materials kept their structural integrity during the culture period while the collagen foams contracted greatly. Further, the total protein production after 4 weeks in culture was found to be twice as high (190 +/- 10%) in the P(HB-9% HV) foam than in the collagen foam. Porous P(HB-HV) materials appear to be adequate polymeric substrates for cell cultures. However, further evaluations are still required to confirm such preliminary results.
一种天然聚（羟基丁酸酯 - 共 - 9%羟基戊酸酯）共聚酯通过盐析/溶剂浇铸法与预先过筛的氯化钠盐一起加工成三维多孔泡沫结构。将实验室制备的聚（羟基丁酸酯 - 9%羟基戊酸酯）泡沫和商用胶原海绵切成小矩形标本，进行灭菌处理，先用乙醇预湿，再用杜尔贝科改良伊格尔培养基 + 10%血清培养基冲洗，然后接种从犬前交叉韧带分离的成纤维细胞。在37℃（5%二氧化碳）孵育0至35天，将成纤维细胞培养到这种多孔基质中。结果表明，聚（羟基丁酸酯 - 羟基戊酸酯）维持的细胞增殖速率与胶原海绵中观察到的相似，至少持续35天，培养第28天细胞密度达到最大值。另一方面，聚（羟基丁酸酯 - 羟基戊酸酯）材料在培养期间保持其结构完整性，而胶原泡沫则大幅收缩。此外，培养4周后，聚（羟基丁酸酯 - 9%羟基戊酸酯）泡沫中的总蛋白产量比胶原泡沫中的高两倍（190±10%）。多孔聚（羟基丁酸酯 - 羟基戊酸酯）材料似乎是用于细胞培养的合适聚合物基质。然而，仍需要进一步评估来证实这些初步结果。
