Shotkoski F, Lee H J, Zhang H G, Jackson M B, ffrench-Constant R H
Department of Entomology, University of Wisconsin-Madison 53706.
Insect Mol Biol. 1994 Nov;3(4):283-7. doi: 10.1111/j.1365-2583.1994.tb00179.x.
We are interested in cloning insecticide resistance genes from vector mosquitos for use as selectable markers in their genetic transformation. As a first step towards this goal, we here report the functional homomultimeric expression of a gamma-aminobutyric acid (GABA) receptor subunit gene, Resistance to dieldrin (Rdl), from the yellow fever mosquito Aedes aegypti in baculovirus-infected insect cell lines. Replacement of alanine296 with a serine leads to approximately 100-fold insensitivity to picrotoxin as previously observed in Drosophila. This shows not only that the mosquito GABA receptor cDNA is functional but also that it can be simply mutated to resistance. Strategies for incorporation of this cDNA into a minigene for the genetic transformation of mosquitoes are discussed.
我们感兴趣的是从媒介蚊子中克隆抗杀虫剂基因，用作其遗传转化中的选择标记。作为实现这一目标的第一步，我们在此报告杆状病毒感染的昆虫细胞系中，来自黄热病蚊子埃及伊蚊的γ-氨基丁酸（GABA）受体亚基基因——狄氏剂抗性（Rdl）基因的功能性同多聚体表达。将丙氨酸296替换为丝氨酸会导致对苦味毒产生约100倍的不敏感性，这与先前在果蝇中观察到的情况一致。这不仅表明蚊子GABA受体cDNA具有功能，还表明它可以简单地发生突变产生抗性。本文讨论了将该cDNA整合到用于蚊子遗传转化的小基因中的策略。
