Buiting A M, Van Rooijen N
Dept. of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
J Drug Target. 1994;2(5):357-62. doi: 10.3109/10611869408996810.
To study the role of macrophages in immune and non-immune defence mechanisms, a new technique to eliminate macrophages has been developed. This technique uses the capability of macrophages to ingest and digest particulate compounds. As particulate compound liposomes with entrapped clodronate are used. Macrophages will ingest these liposomes and after fusion of their endosomes with their lysosomes the bilayers are disrupted under influence of phospholipases and the clodronate is released into the cytoplasm. If the intracellular concentration of free clodronate reaches sufficiently high values, the macrophage will die. The applications of the approach and some of the results obtained up to now using this macrophage 'suicide' technique are discussed.
为研究巨噬细胞在免疫和非免疫防御机制中的作用，已开发出一种消除巨噬细胞的新技术。该技术利用巨噬细胞摄取和消化颗粒化合物的能力。所使用的颗粒化合物是包裹有氯膦酸盐的脂质体。巨噬细胞会摄取这些脂质体，其内涵体与溶酶体融合后，双层膜在磷脂酶的作用下被破坏，氯膦酸盐释放到细胞质中。如果游离氯膦酸盐的细胞内浓度达到足够高的值，巨噬细胞就会死亡。本文讨论了该方法的应用以及迄今为止使用这种巨噬细胞“自杀”技术所获得的一些结果。
