Arstila T P, Toivanen P, Lassila O
Department of Medical Microbiology, Turku University, Finland.
Cell Immunol. 1995 Apr 15;162(1):74-9. doi: 10.1006/cimm.1995.1053.
We have studied the reactivity of chicken T cells to mycobacterial antigens. Neither peripheral blood nor splenic lymphocytes isolated from unprimed chickens proliferated in response to mycobacterial antigens (mycobacterial sonicate, purified protein derivative, or recombinant 65-kDa heat-shock protein HSP65). After immunization with complete Freund's adjuvant (CFA) a strong response appeared, and a transient increase of peripheral blood gamma delta T cells was observed. Analysis of spleen cells isolated from CFA-primed chickens showed that both alpha beta and gamma delta T cells were activated by the mycobacterial antigens, apparently at equal levels. Both subsets also responded to HSP65, but no preference of gamma delta T cells to respond to it or any of the other mycobacterial antigens was observed. These results indicate that although HSP65 is an important mycobacterial antigen, it is not dominant in the chicken gamma delta T cell repertoire. Moreover, the results show a clear distinction between the naive and primed repertoire of gamma delta T cells.
我们研究了鸡T细胞对分枝杆菌抗原的反应性。从未经免疫的鸡中分离出的外周血淋巴细胞和脾淋巴细胞均不会因分枝杆菌抗原（分枝杆菌超声裂解物、纯化蛋白衍生物或重组65 kDa热休克蛋白HSP65）而增殖。用完全弗氏佐剂（CFA）免疫后出现强烈反应，并观察到外周血γδT细胞短暂增加。对从经CFA免疫的鸡中分离出的脾细胞进行分析表明，αβ和γδT细胞均被分枝杆菌抗原激活，且激活水平明显相同。两个亚群也对HSP65有反应，但未观察到γδT细胞对其或任何其他分枝杆菌抗原的反应存在偏好。这些结果表明，尽管HSP65是一种重要的分枝杆菌抗原，但它在鸡γδT细胞库中并不占主导地位。此外，结果显示出γδT细胞的天然库和免疫后库之间存在明显差异。
