类风湿关节炎患者滑液单核细胞对分枝杆菌65kDa热休克蛋白的增殖反应及其与HLA - DR +γδ + T细胞的关联。
Proliferative response of synovial fluid mononuclear cells of patients with rheumatoid arthritis to mycobacterial 65 kDa heat shock protein and its association with HLA-DR+.gamma delta+ T cells.
作者信息
Kogure A, Miyata M, Nishimaki T, Kasukawa R
机构信息
Department of Internal Medicine II, Fukushima Medical College, Japan.
出版信息
J Rheumatol. 1994 Aug;21(8):1403-8.
OBJECTIVE
The proliferative response of mononuclear cells (MNC) in synovial fluid (SF) and peripheral blood (PB) of patients with rheumatoid arthritis (RA) to mycobacterial 65 kDa heat shock protein (HSP65) was tested and the response compared with the percentage of HLA-DR+.gamma delta+ T cells in lymphocytes of SFMNC or PBMNC:
METHODS
Proliferative response of MNC was measured by means of 3H-thymidine incorporation and expressed by means of the stimulation index. Percentage of HLA-DR+.gamma delta+ T cells in lymphocytes was measured by means of the 2-color flow cytometry method.
RESULTS
Higher response of SFMNC than PBMNC to HSP65 was noted in 14 of 19 patients with RA. Stimulation indexes of RA-SFMNC correlated significantly with HLA-DR+.gamma delta+ T cell percentage in lymphocytes.
CONCLUSION
In the SF of patients with RA, an accumulation of HSP65 reactive and HLA-DR+.gamma delta+ T cells was noted.
目的
检测类风湿关节炎（RA）患者滑液（SF）和外周血（PB）中单核细胞（MNC）对分枝杆菌65 kDa热休克蛋白（HSP65）的增殖反应，并将该反应与SF - MNC或PB - MNC淋巴细胞中HLA - DR +.γδ + T细胞的百分比进行比较。
方法
通过3H - 胸腺嘧啶核苷掺入法测定MNC的增殖反应，并以刺激指数表示。采用双色流式细胞术方法测定淋巴细胞中HLA - DR +.γδ + T细胞的百分比。
结果
19例RA患者中有14例显示SF - MNC对HSP65的反应高于PB - MNC。RA - SF - MNC的刺激指数与淋巴细胞中HLA - DR +.γδ + T细胞百分比显著相关。
结论
在RA患者的滑液中，发现了HSP65反应性和HLA - DR +.γδ + T细胞的聚集。
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