Chen J J, Tsai S C, Lu C C, Lee K Y, Chien C H, Tsai C L, Wang P S, Chien E J
Department of Physiology, National Yang-Ming University, Taipei, Taiwan, Republic of China.
Chin J Physiol. 1994;37(3):161-3.
The effect of human calcitonin (hCT) on the secretion of luteinizing hormone (LH) was examined in castrated rats. Male rats were orchiectomized (Orch) 2 weeks before they were treated with a single bolus injection of gonadotropin-releasing hormone (GnRH, 1 microgram/kg of body weight), hCT (3.4 ng/kg), or the combination of GnRH and hCT through a right jugular vein catheter. Blood samples (0.5 ml each time) were collected immediately before (0-min) and then at 15, 30, 60, 120 and 180 min after hormone injection. Plasma LH concentration was measured with a radioimmunoassay technique. Plasma calcium concentration was determined in an automatic analyzer. hCT significantly diminished both the basal and GnRH-stimulated levels of plasma LH without any relation to the reduced plasma levels of calcium. These results clearly demonstrated a plasma calcium-unrelated inhibitory effect of hCT on LH secretion.
在去势大鼠中研究了人降钙素（hCT）对促黄体生成素（LH）分泌的影响。雄性大鼠在通过右颈静脉导管接受单次大剂量注射促性腺激素释放激素（GnRH，1微克/千克体重）、hCT（3.4纳克/千克）或GnRH与hCT的组合治疗前2周进行去势手术（Orch）。在激素注射前（0分钟）以及注射后15、30、60、120和180分钟立即采集血样（每次0.5毫升）。采用放射免疫分析技术测定血浆LH浓度。用自动分析仪测定血浆钙浓度。hCT显著降低了血浆LH的基础水平和GnRH刺激水平，且与血浆钙水平降低无关。这些结果清楚地表明hCT对LH分泌具有与血浆钙无关的抑制作用。
