Isobe H, Sridhar K S, Doria R, Cohen F, Raub W A, Saldana M, Krishan A
Department of Radiation Oncology, University of Miami School of Medicine, Florida, USA.
Cytometry. 1995 Jan 1;19(1):86-91. doi: 10.1002/cyto.990190111.
DNA ploidy of pepsin digested preparations of 48 paraffin-embedded specimens from 19 patients with histologically confirmed malignant mesothelioma was determined by laser flow cytometry. Eight of the 19 tumors (42%) were diploid and 11 (58%) were aneuploid. Of the aneuploid tumors, only one showed multiploidy. The median survival time of the patients with diploid tumors was 19, 16, and 14 months from the onset of symptoms, diagnosis, and treatment, respectively. The median survival in patients with aneuploid tumors was 8, 7, and 7 months from the onset of first symptoms, diagnosis, and treatment. Thus, patients with diploid tumors lived longer than patients with aneuploid tumors. These results suggest that DNA ploidy analysis may be of prognostic value in malignant mesothelioma.
通过激光流式细胞术测定了19例经组织学确诊为恶性间皮瘤患者的48个石蜡包埋标本经胃蛋白酶消化后的DNA倍性。19个肿瘤中有8个（42%）为二倍体，11个（58%）为非整倍体。在非整倍体肿瘤中，只有一个显示多倍体。二倍体肿瘤患者从症状出现、诊断和治疗开始的中位生存时间分别为19个月、16个月和14个月。非整倍体肿瘤患者从首次症状出现、诊断和治疗开始的中位生存时间为8个月、7个月和7个月。因此，二倍体肿瘤患者的生存期比非整倍体肿瘤患者长。这些结果表明，DNA倍性分析可能对恶性间皮瘤具有预后价值。
