豚鼠十二指肠活性碳酸氢盐分泌激动剂的比较活性

Comparative activities of agonists of active duodenal bicarbonate secretion in the guinea pig.

作者信息

Odes H S, Muallem R, Reimer R, Schwenk M, Beil W, Sewing K F

机构信息

Gastroenterology Institute, Soroka Medical Center, Beer Sheva, Israel.

出版信息

Digestion. 1994;55(6):410-6. doi: 10.1159/000201174.

DOI:10.1159/000201174

PMID:7705554

Abstract

The comparative activity of agonists of duodenal bicarbonate secretion was studied in the anesthetized guinea pig, where the duodenal lumen was perfused with 24 mmol/l NaHCO3 to ensure active secretion of bicarbonate. Agonists were infused alone and in combination. Dibutyryl 3',5'-cyclic adenosine monophosphate, vasoactive intestinal polypeptide (VIP) and prostaglandin E2 (PGE2) were strong stimulants of bicarbonate secretion. Theophylline, dibutyryl 3',5'-cyclic guanosine monophosphate, glucagon and prostaglandin F2 alpha (PGF2 alpha) were weaker agonists, and secretin had no effect. Combinations of any two of VIP, PGE2 and glucagon depressed bicarbonate secretion, whereas combinations of PGE2 and PGF2 alpha, VIP and PGE2, and glucagon and PGF2 alpha increased bicarbonate secretion. The data indicate that cAMP and other secondary messengers may mediate duodenal bicarbonate secretion.

摘要

在麻醉的豚鼠身上研究了十二指肠碳酸氢盐分泌激动剂的相对活性，在该实验中，十二指肠腔内灌注24 mmol/l的NaHCO₃以确保碳酸氢盐的主动分泌。激动剂单独或联合注入。二丁酰3',5'-环磷酸腺苷、血管活性肠肽（VIP）和前列腺素E2（PGE2）是碳酸氢盐分泌的强刺激剂。茶碱、二丁酰3',5'-环磷酸鸟苷、胰高血糖素和前列腺素F2α（PGF2α）是较弱的激动剂，而促胰液素没有作用。VIP、PGE2和胰高血糖素中任意两者的组合会抑制碳酸氢盐分泌，而PGE2和PGF2α、VIP和PGE2以及胰高血糖素和PGF2α的组合会增加碳酸氢盐分泌。数据表明，环磷酸腺苷（cAMP）和其他第二信使可能介导十二指肠碳酸氢盐分泌。