Jansen R, Briaire J, Kamp E M, Gielkens A L, Smits M A
Department of Molecular Biology, DLO-Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands.
FEMS Microbiol Lett. 1995 Feb 15;126(2):139-43. doi: 10.1111/j.1574-6968.1995.tb07407.x.
Actinobacillus pleuropneumoniae shows synergistic haemolysis when cocultured with Staphylococcus aureus on blood agar plates. This CAMP effect has been attributed to a discrete CAMP factor, but also to the A. pleuropneumoniae-RTX-toxins I, II, and III. We examined the CAMP effect of recombinant Escherichia coli strains that secreted each of these toxins, and of A. pleuropneumoniae mutant strains that were devoid of one or more these toxins. We found that the E. coli strains were CAMP positive, whereas the A. pleuropneumoniae strain devoid of functional toxin genes was CAMP negative. This demonstrated that the CAMP effect of A. pleuropneumoniae is caused by the toxins and that no CAMP factor per se exists.
胸膜肺炎放线杆菌与金黄色葡萄球菌在血琼脂平板上共培养时表现出协同溶血作用。这种CAMP效应一直被归因于一种离散的CAMP因子，但也归因于胸膜肺炎放线杆菌的RTX毒素I、II和III。我们检测了分泌这些毒素的重组大肠杆菌菌株以及缺失一种或多种这些毒素的胸膜肺炎放线杆菌突变菌株的CAMP效应。我们发现大肠杆菌菌株CAMP试验呈阳性，而缺乏功能性毒素基因的胸膜肺炎放线杆菌菌株CAMP试验呈阴性。这表明胸膜肺炎放线杆菌的CAMP效应是由毒素引起的，且不存在单独的CAMP因子。
