P 因子插入果蝇基因内含子导致的异常剪接和转录终止。
Aberrant splicing and transcription termination caused by P element insertion into the intron of a Drosophila gene.
作者信息
Horowitz H, Berg C A
机构信息
Department of Genetics, University of Washington, Seattle 98195.
出版信息
Genetics. 1995 Jan;139(1):327-35. doi: 10.1093/genetics/139.1.327.
Abstract
Insertional mutagenesis screens using the P[lacZ, rosy+] (PZ) transposable element have provided thousands of mutant lines for analyzing genes of varied function in the fruitfly, Drosophila melanogaster. As have been observed with other P elements, many of the PZ-induced mutations result from insertion of the P element into the promoter or 5' untranslated regions of the affected gene. We document here a novel mechanism for mutagenesis by this element. We show that sequences present within the element direct aberrant splicing and termination events that produce a mRNA composed of 5' sequences from the mutated gene (in this case, pipsqueak) and 3' sequences from within the P[lacZ, rosy+] element. These truncated RNAs could yield proteins with dominant mutant effects.
摘要
使用P[lacZ, rosy+]（PZ）转座因子进行的插入诱变筛选，已经为分析黑腹果蝇中各种功能的基因提供了数千个突变品系。正如在其他P因子中所观察到的那样，许多PZ诱导的突变是由于P因子插入到受影响基因的启动子或5'非翻译区所致。我们在此记录了该因子诱变的一种新机制。我们表明，该因子内存在的序列会导致异常剪接和终止事件，从而产生一种mRNA，其由来自突变基因（在这种情况下为pipsqueak）的5'序列和来自P[lacZ, rosy+]因子内的3'序列组成。这些截短的RNA可能产生具有显性突变效应的蛋白质。
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