Siegel V, Jongens T A, Jan L Y, Jan Y N
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
Development. 1993 Dec;119(4):1187-202. doi: 10.1242/dev.119.4.1187.
We have identified a new member of the posterior group of genes, which we call pipsqueak. We show that pipsqueak acts after the establishment of the oskar posterior anchor but before the localization of vasa protein during oogenesis. Characterization of multiple alleles at the pipsqueak locus shows that pipsqueak, like vasa, is required for early stages of oogenesis, including but not limited to formation of the egg chamber and progression through Stage 6 of oogenesis. Genetic interaction studies suggest that pipsqueak acts at least partially through vasa; molecular studies indicate that pipsqueak affects vasa level in the ovary. We compare vasa and pipsqueak mutant phenotypes in order to determine whether pipsqueak acts solely through vasa, and present a model for the role of pipsqueak in posterior pattern formation.
我们鉴定出了一组后部基因中的一个新成员，我们将其命名为“小不点儿”。我们发现，“小不点儿”基因在卵子发生过程中，于osk基因后部锚定建立之后、vasa蛋白定位之前发挥作用。对“小不点儿”基因座上多个等位基因的特征分析表明，与vasa基因一样，“小不点儿”基因在卵子发生的早期阶段是必需的，包括但不限于卵室的形成以及卵子发生第6阶段的进程。遗传相互作用研究表明，“小不点儿”基因至少部分通过vasa基因发挥作用；分子研究表明，“小不点儿”基因会影响卵巢中vasa蛋白的水平。我们比较了vasa基因和“小不点儿”基因的突变体表型，以确定“小不点儿”基因是否仅通过vasa基因发挥作用，并提出了一个关于“小不点儿”基因在后部模式形成中作用的模型。
