Gonzalez V H, Hu L K, Theodossiadis P G, Flotte T J, Gragoudas E S, Young L H
Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School 02114, USA.
Invest Ophthalmol Vis Sci. 1995 Apr;36(5):871-8.
To evaluate the effectiveness of photodynamic therapy with chloroaluminum sulfonated phthalocyanine in the treatment of pigmented choroidal melanomas in a rabbit model.
Pigment containing B16F10 murine melanoma cells were implanted transclerally into the subchoroidal space of 28 immunosuppressed New Zealand albino rabbits. The animals were treated with daily injections of cyclosporine and were followed up until tumors at least 2 mm in height were detected by ultrasonography. Twenty-four hours after the intravenous injection of chloroaluminum sulfonated phthalocyanine (CASPc, 5 mg/kg), tumors were irradiated at 675 nm through an argon-pumped dye laser at estimated total light doses of 25 to 70 J/cm2. Control animals were treated with light only or photosensitizer only. The animals were followed up for 4 1/2 to 8 weeks with regular fundus examinations.
Twenty tumor-bearing rabbits were treated with light and dye. The tumor regressed in 12 animals. Five of these animals were followed up for at least 4 1/2 weeks and the other seven for 8 weeks after treatment. At light doses under 40 J/cm2, tumor regrowth was observed in five animals within 10 days of treatment. In all control groups, the tumor-bearing eyes were filled with tumor cells by the third week after implantation. Histologic examination of tumors treated with photosensitizer and light revealed prominent vascular damage early after treatment that resulted in vascular occlusion. Tumor necrosis was evident within 24 hours of treatment.
Results suggest that photodynamic therapy may have a role in the treatment of pigmented choroidal melanomas.
评估磺化铝酞菁光动力疗法对兔模型中色素性脉络膜黑色素瘤的治疗效果。
将含色素的B16F10小鼠黑色素瘤细胞经巩膜植入28只免疫抑制的新西兰白化兔的脉络膜下间隙。每日给动物注射环孢素并进行随访，直至超声检查发现高度至少为2mm的肿瘤。静脉注射磺化铝酞菁（CASPc，5mg/kg）24小时后，通过氩泵浦染料激光以675nm波长照射肿瘤，估计总光剂量为25至70J/cm²。对照动物仅接受光照或仅接受光敏剂治疗。通过定期眼底检查对动物进行4.5至8周的随访。
20只荷瘤兔接受了光照和染料治疗。12只动物的肿瘤消退。其中5只动物在治疗后至少随访4.5周，另外7只随访8周。在光剂量低于40J/cm²时，5只动物在治疗后10天内观察到肿瘤复发。在所有对照组中，荷瘤眼在植入后第三周充满肿瘤细胞。对接受光敏剂和光照治疗的肿瘤进行组织学检查发现，治疗后早期血管损伤明显，导致血管闭塞。治疗后24小时内肿瘤坏死明显。
结果表明光动力疗法可能在色素性脉络膜黑色素瘤的治疗中发挥作用。
