Hu D N, McCormick S A, Orlow S J, Rosemblat S, Lin A Y, Wo K
Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary, NY 10003, USA.
Invest Ophthalmol Vis Sci. 1995 Apr;36(5):931-8.
To study melanogenesis by cultured human uveal melanocytes, and the relationship between melanin production by uveal melanocytes in vitro with the degree of iris pigmentation in vivo.
Melanin content, melanin production, and tyrosinase activity of cultured uveal melanocytes derived from eyes of various iris color were measured at different stages of cultivation.
Cultured uveal melanocytes maintained a constant level of melanin content, expressed tyrosinase activity, and produced measurable amounts of melanin in vitro. Melanosomes in different stages were seen ultrastructurally. Melanin production correlated directly with the degree of iris pigmentation of the eyes from which the uveal melanocytes were isolated. Tyrosinase activity of cultured uveal melanocytes from black versus white donors was significantly different, but, among white donors, there was no correlation with iris pigmentation or with melanin production in vitro.
Cultured uveal melanocytes can produce melanin in vitro. Cultured uveal melanocytes isolated from eyes of different iris color maintained their inherent capacity for melanogenesis. Therefore, cultured uveal melanocytes are an excellent model system for studying melanogenesis in uveal melanocytes in vitro.
研究培养的人葡萄膜黑色素细胞的黑色素生成，以及葡萄膜黑色素细胞在体外产生黑色素与体内虹膜色素沉着程度之间的关系。
在培养的不同阶段，测量来自不同虹膜颜色眼睛的培养葡萄膜黑色素细胞的黑色素含量、黑色素生成和酪氨酸酶活性。
培养的葡萄膜黑色素细胞保持恒定的黑色素含量水平，表达酪氨酸酶活性，并在体外产生可测量的黑色素量。超微结构观察到不同阶段的黑素小体。黑色素生成与分离葡萄膜黑色素细胞的眼睛的虹膜色素沉着程度直接相关。来自黑人与白人供体的培养葡萄膜黑色素细胞的酪氨酸酶活性有显著差异，但在白人供体中，与虹膜色素沉着或体外黑色素生成均无相关性。
培养的葡萄膜黑色素细胞可在体外产生黑色素。从不同虹膜颜色眼睛分离的培养葡萄膜黑色素细胞保持其固有的黑色素生成能力。因此，培养的葡萄膜黑色素细胞是体外研究葡萄膜黑色素细胞黑色素生成的优良模型系统。
