在苯丙氨酸消旋化方面存在缺陷的短杆菌肽S合成酶1的突变基因与野生型基因具有相同的序列。

Mutant genes of gramicidin S synthetase 1 defective in phenylalanine racemization have the same sequence as the wild gene.

作者信息

Hori K, Saito F, Tokita K, Kurotsu T, Kanda M, Saito Y

机构信息

Department of Biochemistry, Hyogo College of Medicine.

出版信息

J Biochem. 1994 Dec;116(6):1202-4. doi: 10.1093/oxfordjournals.jbchem.a124663.

DOI:10.1093/oxfordjournals.jbchem.a124663

PMID:7706207

Abstract

Mutant grs1 genes were cloned and sequenced from the Bacillus brevis Nagano BI-4, C-3, E-1, and E-2 strains, which produce defective gramicidin S synthetase 1 (GS1), lacking racemase activity. Surprisingly, these mutant genes had entirely the same sequence as that of the wild type gene. These mutant strains also produce defective gramicidin S synthetase 2 (GS2), lacking 4'-phosphopantetheine, a prosthetic group of this enzyme. The participation of this group in phenylalanine racemization is suggested.

摘要

从短芽孢杆菌长野BI-4、C-3、E-1和E-2菌株中克隆并测序了突变型grs1基因，这些菌株产生有缺陷的短杆菌肽S合成酶1（GS1），缺乏消旋酶活性。令人惊讶的是，这些突变基因的序列与野生型基因完全相同。这些突变菌株还产生有缺陷的短杆菌肽S合成酶2（GS2），缺乏4'-磷酸泛酰巯基乙胺，该酶的一个辅基。提示该基团参与苯丙氨酸消旋化。